African Health Sciences. Vol 19, No 2 (2019):2008-2020

Epidemiology of resistance and phenotypic characterization of carbapenem resistance mechanisms in Klebsiella pneumoniae isolates at Sahloul University Hospital-Sousse, Tunisia
Aziza Messaoudi, Wejdène Mansour, Nedia Jaidane, Chrifa Chaouch, Noureddine Boujaâfar, Olfa Bouallègue

Objective: To assess the prevalence of ESBL producing and carbapenem resistant Klebsiella pneumoniae isolated from in-come? and out-come patients at Sahloul-university hospital.Methods: A retrospective study over a 3 years period (January 2012 and December 2014) focused on 2160 strains of Klebsiella? pneumoniae. Statistical analysis was carried out using SPSS program. ESBL detection was performed using a double disc diffusion? method and carbapenemase detection was realized by Rosco-Disk kit.Results: A total of 2160 Klebsiella pneumoniae strains were isolated during the period of the study, 26.2% (n=566) were ESBL-producers? and 15.8% (n=342) showed resistance to carbapenem. The wards most affected by these strains were basically urology? and intensive care units. Eighty four percent of studied strains (203/241) were resistant to temocillin, which correlate with the? production of a class D (OXA-48-like) carbapenemase and 7% (17/241) showed sensitivity to EDTA and dipicolinic acid, which? indicate the production of metallo-enzyme. The rate of resistance to colistin remains low.Conclusion: Resistance of Enterobacteriaceae, including K. pneumoniae, to third generation cephalosporins (3rd GC) and carbapenem? through the mechanism of ESBL and carbapenemases production is becoming increasingly worrying. This suggests a morerational use of antibiotics, as well as the rigorous application of hygiene measurement.Keywords: Klebsiella pneumoniae, epidemiology, ESBL, carbapenemase, phenotypic screening.

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